PKC-alpha Activation Kit - 5 x 96

The PKCa Activation HCS Reagent Kit comprises optimized and qualified high-content screening (HCS)-grade fluorescence reagents and an automation-compatible protocol for the quantification of PKCa translocation. Cells growing on standand high-density microplates are stimulated, fixed and immunofluorescently labeled to identify PKCa (Figure 1). The kit also includes a cell membrane marker so co-localization of PKCa at the cell membrane may be quantified. Based on indirect immunofluorescence detection of the translocation of PKCa between cytoplasm and plasma membrane, this kit may be used to screen for inhibitors, activators, or modulators of PKCa. Application:Used for Protein Kinase C Alpha (PKCa). Method: Species: Storage: