Rabbit Alpha-2 Macroglobulin ELISA Kit 1 Kit (96 Wells) - 1 Kit

The principle of the double antibody sandwich ELISA is represented in Figure 1.  In this assay the alpha 2-macroglobulin present  in  the  sample  reacts  with  the  anti-A2M  antibodies,  which  have  been  adsorbed  to  the  surface  of  polystyrene microtiter  wells.    After  the  removal  of  unbound  sample  proteins  by  washing,  anti-A2M  antibodies  conjugated  with horseradish  peroxidase  (HRP),  are  added.    This  HRP-conjugated  antibody  forms  a  complex  with  the  previously  bound alpha  2-macroglobulin.    Following  another  washing  step,  the  enzyme  bound  to  the  immunosorbent  is  assayed  by  the addition of a chromogenic substrate, 3,3,5,5-tetramethylbenzidine (TMB).  The quantity of bound enzyme is proportional to the concentration of alpha 2-macroglobulin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of alpha 2-macroglobulin in the test sample.  The quantity of alpha 2-macroglobulin in the test sample can be interpolated from the calibration curve constructed from the calibrators, and corrected for sample dilution. Application: Method:ELISA Species: Storage: