pNPP Phosphatase Assay Kit - 500 assays

Para-nitrophenyl phosphate (pNPP) is a chromogenic substrate for most phosphatases such as alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases. The reaction yields paranitrophenol, which becomes an intense yellow soluble product under alkaline conditions and can be conveniently measured at 405nm on a spectrophotometer. This homogeneous âmix-andmeasureâ assay involves simply adding a single reagent to the phosphatase, and measuring the product formation using any absorbance reader. The assay can be conveniently performed in cuvettes, tubes or multi-well plates at either room temperature or 37°C. The kit reagents have been optimised for long shelf life and maximum reproducibility. The reagents are compatible with all liquid handling systems, and bulk reagents are available for high-throughput screening of phosphatase inhibitors. Application:1. Enzyme Activity Assay and Quality Control for phosphatase production.2. Characterization of Kinetics of phosphatase reaction.3. Drug Discovery: high-throughput screen for phosphatase inhibitors. Method: Species: Storage:Store at -20°C Stability:Stable for 12 months from manufacture. Sensitivity: Time: