SensoLyte 620 HIV Protease Assay Kit *Fluorimetric* - 1 kit

SensoLyte⢠620 HCV Assay Kit uses a new FRET peptide substrate that incorporates HiLyte Fluor⢠TR (fluorophore) and QXL⢠610 (quencher) for continuous measurement of the enzyme activities. In the intact FRET peptide, the fluorescence of Hylite Fluor⢠TR is quenched by QXL⢠610. Upon cleavage of the FRET peptide by HIV protease, the fluorescence of Hylite Fluor⢠TR is recovered and can be continuously monitored at excitation/emission = 590 nm/620 nm. With superior fluorescence quantum yield and longer emission wavelength, HiLyte Fluor⢠TR/QXL⢠610-based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components. This FRET peptide also provides better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format. The kit contains: FRET peptide substrate (fluorogenic indicator, Ex/Em=590/610nm upon cleavage), Assay buffer, HIV protease inhibitor, Fluorescence reference standard for calibration, A detailed protocol Application:F Method: Species: Storage:-20°C