SREBP-2 Cell-Based Translocation Assay Kit - 96 Well

Lipid homeostasis in vertebrate cells is regulated by a family of basic helix-loop-helix (HLH) transcription factors called sterol regulatory element binding proteins (SREBPs). SREBPs directly activate the expression of over 30 genes involved in both the synthesis and uptake of cholesterol, fatty acids, triglycerides, and phospholipids. SREBP-2 regulates cholesterol synthesis by activating the transcription of genes for HMG-CoA reductase and other enzymes of the cholesterol synthetic pathway. It is also involved in activating genes required to generate NADPH, which is consumed at multiple stages in cholesterol biosynthesis. SREBP-2 is ubiquitously expressed. Cayman`s SREBP-2 Cell-Based Translocation Assay Kit provides the tools needed to study SREBP-2 movement within whole cells. The kit contains a highly specific SREBP-2 primary antibody together with a DyLightTM (trademarked by Pierce Biotechnology Inc.) conjugated secondary antibody in a ready to use format. Also included as a positive control is a cholesterol trafficking inhibitor, U18666A, which has been shown to activate SREBP-2 translocation into nuclei by scientists at Cayman Chemical Company.Application/Cell-Based Assay, Chemical Class/Assay Kits, Research Area/Atherosclerosis, Research Area/Diabetes, Research Area/Metabolic Syndrome, Research Area/ObesitySynonyms :Sterol Regulatory Element-Binding Protein-2 Cell-Based Translocation Assay Kit